| Microbiome and mycobiome analyses in continuous positive airway pressure devices |
Hyun Jin Min1,2
, Bo-Yun Choi3
, Woo Jun Sul3
, Hyung-Ju Cho4,5
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1Department of Otorhinolaryngology–Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Republic of Korea
2Biomedical Research Institute, Chung-Ang University Hospital
3Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do, Republic of Korea
4Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of Korea
5The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Republic of Korea |
| Correspondence |
Woo Jun Sul ,Tel: +82-31-670-4707, Fax: +82-31-675-3108, Email: sulwj@cau.ac.kr
Hyung-Ju Cho ,Tel: +82 2 2228 3605, Fax: +82 2 393 0580, Email: hyungjucho@yuhs.ac
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Received: June 16, 2024; Revised: August 4, 2024 Accepted: August 12, 2024. Published online: August 13, 2024.
*Hyun Jin Min and Bo-Yun Choi contributed equally to this work. |
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| ABSTRACT |
Objectives
Microorganisms are likely present in continuous positive airway pressure (CPAP) devices used daily. Considering the potential risk of infections among CPAP device users, here we aimed to compare the microbiomes in CPAP devices with those in nasal mucosal samples obtained from corresponding individuals using these devices.
Methods
We conducted a prospective cohort study at tertiary medical institutes. Samples were collected from the tubes and filters of CPAP devices and the nasal mucosa of corresponding individuals using these devices. Microbiomes and mycobiomes were analyzed using 16S ribosomal RNA and internal transcribed spacer region sequencing. Results were compared according to the sampling site and usage duration for each patient.
Results
Overall, 27 paired human nasal mucosa and CPAP samples were analyzed. Bacteria were present in 7 of 27 tubes (29.6%) and 22 of 27 filters (81.5%). Fungi were present in 2 of the 27 tubes (7.4%) and 16 of the 27 filters (59.3%). Actinobacteria and Firmicutes were the predominant phyla among all samples. Fungi were not detected in any of the nasal mucosal samples. However, Basidiomycota and Ascomycota were predominant in the CPAP filters and tube samples. No significant associations were identified among the results according to sampling site and usage duration.
Conclusion
Bacteria or fungi can be detected to some extent in CPAP samples even if the CPAP usage period is short. The association between respiratory infections and these microbiomes or mycobiomes was not investigated. Further research might be required to determine the risk posed by CPAP devices as a microbial contamination source. |
| Keywords:
continuous positive airway pressure device; microbiome; microbial contamination source; mycobiome; obstructive sleep apnea |
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